Fig. 5.
Fig. 5. NF-κB/Rel and IκBα in nuclear and cytoplasmic extracts of day 10 and 14 BFU-E–derived cells. Nuclear and cytoplasmic extracts were prepared from 1.5 × 107 day 10 or 14 BFU-E–derived cells. A total of 20 μg of nuclear or 15 μg of cytoplasmic extract was loaded on each lane of a 10% polyacrylamide gel and Western blotting performed with anti-p50, anti-p52, anti-p65, and anti-IκBα antibodies and ECL. Four experiments were performed with similar results.

NF-κB/Rel and IκBα in nuclear and cytoplasmic extracts of day 10 and 14 BFU-E–derived cells. Nuclear and cytoplasmic extracts were prepared from 1.5 × 107 day 10 or 14 BFU-E–derived cells. A total of 20 μg of nuclear or 15 μg of cytoplasmic extract was loaded on each lane of a 10% polyacrylamide gel and Western blotting performed with anti-p50, anti-p52, anti-p65, and anti-IκBα antibodies and ECL. Four experiments were performed with similar results.

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