ELMO1 regulates thrombus formation and hemostasis in vivo. (A) Survival curves of WT and ELMO1^−/− mice in a pulmonary thromboembolism model. Time to cessation of respiration was recorded after IV administration of 400 μg/kg collagen and 60 μg/kg epinephrine or phosphate-buffered saline. WT, n = 7; ELMO1^−/−, n = 6. (B) Representative carotid artery blood flow tracings (i) and average time to occlusion (ii) from WT and ELMO1^−/− mice. A carotid artery was isolated from the indicated mice and was exposed to 7.5% FeCl₃ injury for 90 seconds. Data are presented as mean ± SE. WT, n = 12; ELMO1^−/−, n = 14. Statistical analysis was performed using a Mann-Whitney U test (*P < .05). (C) Tail bleeding times of WT and ELMO1^−/− mice. The distal 3 mm of the tail was cut, and the tail was immersed in 37°C saline. The time it took for bleeding to stop was recorded. WT, n = 38; ELMO1^−/−, n = 18. Statistical analysis was performed using a Student t test (*P < .05).