Warfarin impairs the BMM. (A) Trichrome stain of the distal femora of a representative control vs a warfarin-treated mouse. Note the dramatic reduction in cancellous bone (green) in warfarin-treated animals compared with vehicle control. Osteoid is stained in pink above the green cancellous bone. The scale bar depicts 100 μm (n = 5). (B-D) Ratios of bone volume/tissue volume (P < .001; t-test, n = 4-5) (B), osteoid volume/bone volume (P = .046; t-test, n = 4-5) (C), and osteoblast surface/bone surface (P = .0008, t-test, n = 4-5) (D) in control (red circles) vs warfarin-treated (blue squares) mice.