![Cyfip1-deficient platelets are unable to form lamellipodia on a fibrinogen-coated surface. (A) Washed platelets were allowed to spread on fibrinogen, following fixation at the indicated time points (n = 3, representative for 3 independent experiments). Scale bar, 5 µm. (B) Quantification of the different spreading phases of fixed platelets after 5, 15, and 30 minutes from differential interference contrast images (Zeiss Axiovert 200 inverted microscope [100×/1.4 oil objective]). (C) Phase abundance of live cells over time (wild type [WT], n = 58; knockout [KO], n = 32). Quantification of (D) platelet size and (E) platelet perimeter (WT, n = 100; KO, n = 58) of all Cyfip1+/+ and Cyfip1−/− platelets present in a visual field after 30-minute spreading. (F) Length and (G) number of filopodia (WT, n = 58; KO, n = 32) of live platelets in phase 2 spreading. Values are mean plus or minus SD. ***P < .001.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/134/25/10.1182_blood.2019002105/7/bloodbld2019002105r1f2.png?Expires=1749969214&Signature=4px~-dlF4bM9hpVj1are~7Fvt0fNaX34fgoloRxvYX83VSs7VOOdV1N5Sai9N~HmR~DqcND~0-6u3Ijg55l3GdrX3mkVxv8Q0lRt1YK2CwORV3PQJ3SIRIKCtJQZhl~PFit7EesFrmxmr5O2LGSl9ZTMxlyKX6ApbVxGreBPWfqLEbxAXg3JdIGA2~~iJgaWGBFrAhSN938tyD5N30t2jEP4rQseHdJxTZImC8OiJFeuDcxVp~1R2E1NPkDPiCeflq5bEdlMWWevUjnhBGztMsx6UHr~DOyIYqqFsgB41hIlA8JAmC-HU2LY7ayuOeLjLdGn7whtvwvVQd-qIV60Qw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cyfip1-deficient platelets are unable to form lamellipodia on a fibrinogen-coated surface. (A) Washed platelets were allowed to spread on fibrinogen, following fixation at the indicated time points (n = 3, representative for 3 independent experiments). Scale bar, 5 µm. (B) Quantification of the different spreading phases of fixed platelets after 5, 15, and 30 minutes from differential interference contrast images (Zeiss Axiovert 200 inverted microscope [100×/1.4 oil objective]). (C) Phase abundance of live cells over time (wild type [WT], n = 58; knockout [KO], n = 32). Quantification of (D) platelet size and (E) platelet perimeter (WT, n = 100; KO, n = 58) of all Cyfip1+/+ and Cyfip1−/− platelets present in a visual field after 30-minute spreading. (F) Length and (G) number of filopodia (WT, n = 58; KO, n = 32) of live platelets in phase 2 spreading. Values are mean plus or minus SD. ***P < .001.