Reduced efficacy of prophylactic FIX_WT treatment in CRM⁺ compared with CRM⁻ mice. SVBA evaluation of hemostasis 7 days after the infusion of 150 IU/kg of FIX_WT in CRM⁻ and CRM⁺ mice. Baseline SVBA in untreated CRM⁻ (black) and CRM⁺ (orange) mice, CRM⁺ mice infused with FIX_WT (green), CRM⁻ mice infused with FIX_WT (light green), and wild-type mice (red). Box plot rendition of the results showing the minimum, first quartile, median (waist), third quartile, and maximum values.