Novel TCR-oncogene translocations with FISH profiles. (A-B) New TCRβ oncogene partner. Bold and thin bars depict the 4q25 or 22q13 and 7q34 chromosomal regions, respectively. Untemplated nucleotides (n diversity) are indicated in lowercase. Nucleotide sequences for the Dβ1, and Jβ gene segments are depicted in italic bold and bold, respectively. Rights panels show a typical FISH metaphase analysis with a normal allele (split spots) and a translocated allele (fused spots) with TCRβ (green) and oncogenes (red) probes. (C) New TCRα/δ oncogene partner. Bold and thin bars depict the 20p11 or 9q21 and 14q11 chromosomal regions, respectively. Untemplated nucleotides (n diversity) are indicated in lowercase. Nucleotide sequences for the Dδ2, Dδ3, and Jδ1 gene segments are depicted in bold italic, dark gray, and bold, respectively. Right panel show a typical FISH analysis on metaphase with a normal allele (split spots) and a translocated allele (fused spots) with TCRα/δ (green) and oncogenes (red) probes. (D) Three-color FISH analysis using a combination of TLX1 (green), GNAQ (yellow), and TCRα/δ (red) probes.