Figure 5
PAR1 and PAR2 differentially affect coagulation protease-mediated infection. HUVECs were incubated with HSV1 (4.5 × 105 vp/mL, TF+/gC+) and thrombin (10nM), FXa (1nM), FVIIa (2.5nM), or FXa/FVIIa (1nM/0.5nM) with control murine IgG (50nM), control IgG plus enzyme, anti-PAR1 (150nM) plus enzyme, or anti-PAR2 (50nM) plus enzyme. The data were corrected for the amount of infection without added protease in the presence of control IgG (n = 4; data are ± SEM). *P ≤ .05 compared with control IgG plus enzyme.
