ERα expression promotes DC development during inflammation in vivo. Using flow cytometry, conventional CD11c⁺ CD11b⁺ DCs (cDCs) were identified in BM of ERα⁺/ERα^−/− BM chimeric mice on days 21 to 24 after reconstitution, a postradiation inflammatory environment. After gating on this DC population, the percentage of cells bearing CD45.1 (WT ERα⁺) or CD45.2 (ERα^−/−) was determined. Shown are the (A) relative percentage and (B) number of cDCs derived from WT and ERα^−/− donor BM in individual mice (n = 13), with the mean and SEM values indicated by the bars. Significant differences in the percentage and numbers of WT and ERα^−/− DCs were calculated using paired t tests. The total number of BM cells in individual mice varied between 4 × 10⁶ and 1 × 10⁷.