Example of a protein analysis of spot 8. (A) Enlarged image of 2D-DIGE showing protein spot 8. Platelets were incubated with control IgG1 (left) or HGP4C9 for GPVI activation (right) in the pH range 4.5 to 6.8. Below, corresponding 3D images of protein spot 8 are shown. (B) Graph view of spot pair 8 in 7 biologic replicates. The log standardized abundance is derived from the normalized spot volume standardized against the in-gel standard of each gel.