Figure 4
Figure 4. DF, DF fractions, and N-mers release 125I-bFGF from low-affinity binding sites on ECM. ECM-coated wells were incubated (3 hours, room temperature) with 125I-bFGF (2.5 × 104/well). The ECM was washed 3 times and incubated (3 hours, room temperature) with increasing concentrations of DF (A and C), DF fractions (A), or N-mers (B). Released radioactivity is expressed as the percentage of total ECM-bound 125I-bFGF and is compared with release by 15 μmol/L SdC28. Each assay was conducted in duplicate, with the differences in duplicate measurements of less than 10%. Identical experiments were repeated 3 times. Maximum release of 125I-bFGF by 2 mol/L NaCl was used as the positive control.

DF, DF fractions, and N-mers release 125I-bFGF from low-affinity binding sites on ECM. ECM-coated wells were incubated (3 hours, room temperature) with 125I-bFGF (2.5 × 104/well). The ECM was washed 3 times and incubated (3 hours, room temperature) with increasing concentrations of DF (A and C), DF fractions (A), or N-mers (B). Released radioactivity is expressed as the percentage of total ECM-bound 125I-bFGF and is compared with release by 15 μmol/L SdC28. Each assay was conducted in duplicate, with the differences in duplicate measurements of less than 10%. Identical experiments were repeated 3 times. Maximum release of 125I-bFGF by 2 mol/L NaCl was used as the positive control.

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