Comparison of antitumor efficacy by free or tumor-loaded αGC. (A) BALB/c mice received 1 × 10⁶ live A20 cells intravenously (day 0) before they were vaccinated with A20/αGC, intravenous injection of αGC (2 μg/mouse) plus A20/veh (A20/veh + αGC), or αGC alone (2 μg/mouse) at day 5 (n=7 per group). (B) Mice were vaccinated with the indicated regimen with nonirradiated A20 cells (live A20/veh, live A20/αGC) on day −7 (n=5 per group). On day 0, all mice received 10⁶ live A20 cells intravenously. Nonvaccinated mice were used as a control (Nil). The survival was monitored daily. P values were calculated in comparison with the nonvaccinated group.