Figure 2
Preventive antitumor activity generated by A20/αGC vaccination. (A) A20 cells were cocultured overnight with 1 μg/mL αGC (A20/αGC) or vehicle (0.5% polysorbate; A20/veh) followed by irradiation (50 Gy). BALB/c mice (n=6-7 per group) were vaccinated with A20/veh, A20/αGC, or left untreated (Nil). After 7 days, all mice received 1 × 106 live A20 cells intravenously, and the survival was checked (day −7). (B) Survivors from panel A were rechallenged with live A20 cells 80 days after primary inoculation (Tumor rechallenge). Age-matched naive mice (n=5) were used as control (Nil). P values were calculated in comparison with the nonvaccinated group. Data shown represent at least 2 independent experiments.
