Organ iron content increases over time in β-thalassemia intermedia. (A) Iron content (μg/mg dry weight), (B) organ weight (g), and (C) total iron (μg) of liver, spleen, kidney, and heart. 2M indicates 2-month-old; 5M, 5-month-old; 12M, 12M-month-old; ■, +/+; ▩, th3/+. Error bars represent SD performed on 3 to 11 independent mice/tissues in duplicate for a total of 90 mice sampled. *P < .05, **P < .01, and ***P < .001 relative to control using the Dunnett multiple comparison test.