Characterization of bone marrow cells from wild-type (+/+), heterozygous (+/−), and serglycin knockout (−/−) mice. (A-B) Giemsa staining of bone marrow cells from wild-type (A) and serglycin^−/− mice (B). Murine neutrophils are characterized by donut-shaped nuclei with increasing lobulation during final maturation; bar, 20 μm. (C-D) Ultrastructure by electron microscopy of neutrophils from wild-type (C) and serglycin^−/− mice (D). Higher magnifications of areas with granules from wild-type (E) and serglycin^−/− neutrophils (F) show the same electron density of granules from the 2 genotypes. (G) Northern blotting with full-length serglycin probe. (H) Ethidium bromide staining of the gel before blotting, for control of equal loading; 5 μg total RNA from bone marrow cells per lane.