Equivalent transformation units of Akata and B95-8 virus demonstrate differential formation of lymphoproliferative diseases in vivo. (A) Survival curve of Akata (n = 9, solid line) and B95-8-infected mice (n = 8, dashed line). P = .001, log-rank test. (B-C) Immunohistochemical staining of spleens from EBV-infected mice. (B) The spleens were stained with hematoxylin and eosin or treated for EBER in situ hybridization. Scale bar, 100 μm. (C) CD68 and CD163 immunohistochemical staining of the spleen. Scale bar, 100 μm. Cells were counted in 16 fields from 3 Akata-infected mice and 20 fields from 3 B95-8-infected mice, respectively. Ratio to total cells were calculated. *P < .01, Mann-Whitney U test. (D) Production of IL-10 was detected by immunohistochemical staining of spleens collected from EBV-infected mice, following the manufacturer’s protocol. Rabbit anti-human IL-10 (LS-B7432) was purchased from LifeSpan BioSciences, Inc. (Seattle, WA). Biopsies were collected from 3 Akata- and B95-8-infected mice. Scale bar, 25 μm.