Figure 5
Figure 5. Loss of Casp9 leads to oligoclonal hematopoiesis after alkylator treatment. FLCs from three Casp9−/− or Casp9+/+ FLCs were each transplanted into 4 lethally-irradiated WT congenic recipients. At 6 and 7 weeks posttransplant, recipient mice were injected with ENU (100 mg/kg) or an equivalent volume of 10% DMSO. At 12 weeks after treatment, donor cells were sorted from the bone marrow and exome sequencing was performed. Somatic single nucleotide variants (SNVs; present in the bone marrow and absent in matched-donor fetal tissue) were identified. SNVs were more frequent and had higher VAFs in ENU-treated vs DMSO-treated Casp9−/− cells. Within each plot, the dot represents the median VAF and the bar indicates the 95% confidence interval. The total number of somatic SNVs detected in each sample is shown below.

Loss of Casp9 leads to oligoclonal hematopoiesis after alkylator treatment. FLCs from three Casp9−/− or Casp9+/+ FLCs were each transplanted into 4 lethally-irradiated WT congenic recipients. At 6 and 7 weeks posttransplant, recipient mice were injected with ENU (100 mg/kg) or an equivalent volume of 10% DMSO. At 12 weeks after treatment, donor cells were sorted from the bone marrow and exome sequencing was performed. Somatic single nucleotide variants (SNVs; present in the bone marrow and absent in matched-donor fetal tissue) were identified. SNVs were more frequent and had higher VAFs in ENU-treated vs DMSO-treated Casp9−/− cells. Within each plot, the dot represents the median VAF and the bar indicates the 95% confidence interval. The total number of somatic SNVs detected in each sample is shown below.

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