Figure 4.
Multilineage engraftment following IUHCT with CD26-inhibited allogeneic BM. E14 BALB/c fetal mice were injected intravenously with 20 × 106 B6GFP BM cells that had been incubated with 5 mM Diprotin A. Peripheral blood from chimeric mice was analyzed at 4 months of age for the presence of GFP cells that expressed CD3 (T cells), B220 (B cells), Gr-1 (granulocytes), and CD11b (macrophages) to assess for donor-cell multilineage engraftment. There was no significant difference in multilineage chimerism levels between control mice and mice injected in utero with CD26-inhibited BM.
