Effect of different priming protocols on secretion or surface exposure of different granule markers, and on the oxidative response to galectin-3. Peripheral blood neutrophils were pretreated as stated in the Materials and Methods section. The top panel (a) shows release into the medium of markers for gelatinase granules (gelatinase), specific granules (gelatinase and vitamin B₁₂–binding protein), and azurophil granules (MPO). The values are given as percent released marker of the total amount in control cells. The middle panel (b) shows the surface exposure of the membrane components CR1 (mobilized from the secretory vesicles) and CR3 (mobilized from secretory vesicles, gelatinase granules, and specific granules), calculated from the mean fluorescence intensity of each cell population and expressed in percent of the value obtained with control cells (□). The lower panel (c) shows the extracellular and intracellular production of superoxide anion in response to galectin-3 (20 μg/mL). The responses are measured as in Fig 2 and are given as chemiluminescence (CL) units in megacounts per minute (Mcpm). Data are given as mean + SD, n = 4. (▧), 22°C; (▪), fMLP; (▨), ionomycin.