Fig. 5.
Fig. 5. Demonstration of proviral integration analysis of in vitro clonogenic progenitor cells derived from retrovirally transduced murine BM cells. Individual colonies were isolated from methylcellulose cultures containing total EGFP-transduced BM cells, FACS-sorted EGFP-negative or EGFP-positive cells, and analyzed by PCR using primers specific for EGFP (EGFP). As a positive control for PCR, primers specific for the murine peripheral cannabinoid receptor (mCb2) were used.58

Demonstration of proviral integration analysis of in vitro clonogenic progenitor cells derived from retrovirally transduced murine BM cells. Individual colonies were isolated from methylcellulose cultures containing total EGFP-transduced BM cells, FACS-sorted EGFP-negative or EGFP-positive cells, and analyzed by PCR using primers specific for EGFP (EGFP). As a positive control for PCR, primers specific for the murine peripheral cannabinoid receptor (mCb2) were used.58 

or Create an Account

Close Modal
Close Modal