Fig. 3. Phosphotyrosine blot of the... | American Society of Hematology

Fig. 3.

$Fig. 3. Phosphotyrosine blot of the nuclear fraction of Jurkat 76.25 cells after MHC-I ligation. Cells (3 × 107) were preincubated with PBS (lane 1) or saturating amounts of anti-β2M Ab (lanes 2 and 3) before exposure to avidin for the indicated time. The separated proteins were immunoblotted with antiphosphotyrosine Ab.$

Phosphotyrosine blot of the nuclear fraction of Jurkat 76.25 cells after MHC-I ligation. Cells (3 × 10⁷) were preincubated with PBS (lane 1) or saturating amounts of anti-β₂M Ab (lanes 2 and 3) before exposure to avidin for the indicated time. The separated proteins were immunoblotted with antiphosphotyrosine Ab.

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