Fig. 6.
Fig. 6. EMSA of CD28RC in OKT3-stimulated CD4 cells derived from unfractionated or CD14-depleted G-PBMCs. A total of 1 × 106/mL unfractionated G-PBMCs and CD14 depleted G-PBMCs were cultured in OKT3-coated T75 culture flasks for 4 hours. One microgram of nuclear extract isolated from CD4 cells derived from either unfractionated (lane 1) or CD14-depleted (lane 2) G-PBMCs was used for EMSA. Results shown are generated from the same sample used in the experiment shown in Fig 5.

EMSA of CD28RC in OKT3-stimulated CD4 cells derived from unfractionated or CD14-depleted G-PBMCs. A total of 1 × 106/mL unfractionated G-PBMCs and CD14 depleted G-PBMCs were cultured in OKT3-coated T75 culture flasks for 4 hours. One microgram of nuclear extract isolated from CD4 cells derived from either unfractionated (lane 1) or CD14-depleted (lane 2) G-PBMCs was used for EMSA. Results shown are generated from the same sample used in the experiment shown in Fig 5.

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