RT-PCR analysis of the DDX10-NUP98 and NUP98-DDX10 chimeric transcripts in four inv(11) patients with de novo or secondary myeloid malignancies. (A) Detection of RT-PCR products by electrophoresis. The DDX10-NUP98 and NUP98-DDX10 chimeric transcripts were amplified with primers DF26 and NR17, and primers NF2 and DR27, respectively (see Fig 1C and D). Pt1, Pt2, Pt3, Pt4: RT-PCR amplified patient RNA samples. Pt1 (−), Pt2 (−): patient 1 and 2 PCR product without reverse transcription. Ct: RT-PCR amplified normal peripheral blood RNA. Arrows indicate the detected fusion transcripts. (B) Sequences of the junctions of the NUP98-DDX10 chimeric transcripts (DDBJ/EMBL/GenBank accession nos. AB000267 and AB000268). Two RT-PCR products (c and d) were sequenced. Nucleotide sequences and predicted amino acid sequences around the junctions are shown. The vertical arrows indicate the junction points of the two genes.