NICD overexpression in ECs rescues the EHT defects in hif-1α and hif-2α morphants. (A-H) Brightfield images of WISH for runx1/cmyb expression in (A-B) 36 hpf control MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos at 28°C, (C-D) control MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock, (E-F) hif-1α MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock, and (G-H) hif-2α MO-injected WT and Tg(hsp70:GAL4;UAS:NICD) embryos after heat shock. (I) Quantification of runx1/cmyb WISH results (A-H), showing the percentages of embryos with WT, increased expression, and decreased expression in each condition. (J-O) Brightfield images of WISH for runx1/cmyb expression in (J-K) 36 hpf control MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos, (L-M) hif-1α MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos, and (N-O) hif-2α MO-injected WT and Tg(fli1a:GAL4;UAS:NICD) embryos (lateral views). (P) Quantification of runx1/cmyb WISH results (J-O), showing the percentages of embryos with WT, increased expression, and decreased expression in each condition. Scale bar, 100 μm.