Figure 3.
Figure 3. Hypoxia is a potent inducer of HSC formation. (A) Schematic representation of the experiment shown in B-H. (B-G) Brightfield images of WISH for (B-C) runx1, (D-E) cmyb, and (F-G) runx1/cmyb expression in 36 hpf WT embryos in normoxia and after hypoxia exposure for 8 hours starting at 28 hpf (lateral views). Scale bar, 100 μm. (H) Quantification of runx1 (B-C), cmyb (D-E), and runx1/cmyb (F-G) WISH results, showing the percentages of embryos with WT and increased expression in each condition. (I) qPCR analysis of runx1 and cmyb mRNA levels in 36 hpf WT embryos in normoxia and after hypoxia exposure for 8 hours starting at 28 hpf (data for whole embryos is shown; data for dissected trunk is provided in supplemental Figure 11). Values represent mean plus SD (n = 3 biological replicates). Ct values for each gene in WT samples in normoxia: rpl13 = 17.25; runx1 = 28.23; cmyb = 24.48. *P < .05 (Student t test).

Hypoxia is a potent inducer of HSC formation. (A) Schematic representation of the experiment shown in B-H. (B-G) Brightfield images of WISH for (B-C) runx1, (D-E) cmyb, and (F-G) runx1/cmyb expression in 36 hpf WT embryos in normoxia and after hypoxia exposure for 8 hours starting at 28 hpf (lateral views). Scale bar, 100 μm. (H) Quantification of runx1 (B-C), cmyb (D-E), and runx1/cmyb (F-G) WISH results, showing the percentages of embryos with WT and increased expression in each condition. (I) qPCR analysis of runx1 and cmyb mRNA levels in 36 hpf WT embryos in normoxia and after hypoxia exposure for 8 hours starting at 28 hpf (data for whole embryos is shown; data for dissected trunk is provided in supplemental Figure 11). Values represent mean plus SD (n = 3 biological replicates). Ct values for each gene in WT samples in normoxia: rpl13 = 17.25; runx1 = 28.23; cmyb = 24.48. *P < .05 (Student t test).

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