SE abates the risk of organ infiltration by NY-ESO-1 redirected T lymphocytes. (A) Hematoxylin-eosin staining of formalin-fixed, paraffin-embedded mouse tissues (liver, kidney, lung, gut, tongue, and ear) from 3 representative animals treated with mock-transduced (left column), transferred (middle column), and SE (right column) cells, respectively. Human lymphocytes displayed larger size when compared with their, albeit minimally present, animal counterpart. Liver involvement occurred mainly under the form of lymphocytic collections around major vessels as well as intraparenchimal nodules of variable size. In the kidney, along with perivascular arrangement, a periglomerular involvement was also encountered. Pulmonary localization was mainly around major vessels and peribronchial in a smaller proportion. In gut and ear, human lymphocytes occurred mostly under the form of few, scattered cells with subepithelial location. Interestingly, in the tongue, human lymphocytes displayed a superficial distribution, immediately beneath or exactly at dermo-epithelial junction, thus recapitulating a “graft-versus-host”–like pattern. The degree of tissue infiltration by human lymphocytes was assessed as described in “Methods” and further confirmed by immunohistochemistry with an anti-human CD3 rabbit monoclonal antibody (B). All pictures were taken at ×200 original magnification.