NOX2 and the development of AML in an in vivo xenograft model. (A) Mice were imaged using bioluminescence weekly to monitor engraftment and disease progression in the animals injected with control and NOX2 KD OCI-AML3-luc cells (B) The survival of NSG mice injected with either control KD or NOX2 KD OCI-AML3-luc cells. (C) Engraftment of AML in bone marrow harvested after the human end point was analyzed by flow cytometry for human CD45 expression. (D) Mitochondrial levels were analyzed in the OCI-AML3-luc pre injection into recipient animals by staining for 15 minutes in 200 mM MitoTracker Green FM. (E) Mitochondrial levels in the purified OCI-AML3-luc population after transplant were also analyzed as in panel D.