Identification of the PD-L1–IGH translocation in sample DL48. (A-B) The positions of the breakpoints in the 2 loci are indicated. The translocation is balanced, resulting in 2 distinct fusion chromosomes. Breakpoint-specific PCR and Sanger sequencing confirm the WGS results. The red box indicates the PCR product with expected size. The 100-bp plus ladder from Life Technologies was used. (C) FISH analysis of sample DL48 shows the colocalization of the probe signals of PD-L1 and IGH. The 5′ end of IGH appears in green, and the 3′ end in red. The probe targeting the 5′ end of the PD-L1/PD-L2 locus was labeled in cyan and the probe targeting the 3′ end in gold.