CCR5 modification is detected in lymphoid and myeloid lineages, including naïve and memory CD4⁺ T cells. Following hematopoietic recovery, large-volume peripheral blood draws were collected from 4 transplanted animals, and the indicated lymphoid and myeloid subsets were enriched. (A) Bead-based positive selection was used to isolate CD4⁺, CD8⁺, CD20⁺, and CD14⁺ subsets. CCR5 disruption in each subset was measured by Illumina MiSeq. (B) Purities of bead-separated subsets from (A) were measured by flow cytometry. Shown are the average and standard deviation for 3 out of 4 animals (an insufficient number of cells were available for this analysis from animal ID Z12161). (C) Fluorescence-activated cell sorting was used to isolate naïve, central memory, and effector memory CD4⁺ T-cell subsets, followed by quantification of CCR5 disruption as in (A). PBMC, peripheral blood mononuclear cells.