Figure 2
Figure 2. Expression of LOX transgene induces MKP expansion. (A) Flow cytometric analysis of megakaryocyte ploidy in Pf4-Loxtg/tg mice compared with matching WT mice. Average ± SD of percentage of cells in each ploidy level is shown (n = 6). (B) Representative flow cytometric analysis of megakaryocyte ploidy. Histograms represent gated CD41+PI+ regions, with CD41 staining megakaryocytes and propidium iodide (PI) staining DNA. (C) Average ± SD of number of acetylcholinesterase-positive (AchE+) cells per 2 × 105 cytospun bone marrow cells in Pf4-Loxtg/tg mice (n = 6) compared with WT mice (n = 8). *P < .01. (D) Average ± SD (n = 6, 6-week-old mice) of percentage of MKPs (Lin−c-Kit+Sca-1−CD150+CD41+) in WT and Pf4-Loxtg/tg mice. (E) Gating strategy for analysis of MKPs. FSC, forward scatter; MKP, megakaryocyte progenitor; SD, standard deviation; SSC, side scatter; Tot BM, total bone marrow.

Expression of LOX transgene induces MKP expansion. (A) Flow cytometric analysis of megakaryocyte ploidy in Pf4-Loxtg/tg mice compared with matching WT mice. Average ± SD of percentage of cells in each ploidy level is shown (n = 6). (B) Representative flow cytometric analysis of megakaryocyte ploidy. Histograms represent gated CD41+PI+ regions, with CD41 staining megakaryocytes and propidium iodide (PI) staining DNA. (C) Average ± SD of number of acetylcholinesterase-positive (AchE+) cells per 2 × 105 cytospun bone marrow cells in Pf4-Loxtg/tg mice (n = 6) compared with WT mice (n = 8). *P < .01. (D) Average ± SD (n = 6, 6-week-old mice) of percentage of MKPs (Linc-Kit+Sca-1CD150+CD41+) in WT and Pf4-Loxtg/tg mice. (E) Gating strategy for analysis of MKPs. FSC, forward scatter; MKP, megakaryocyte progenitor; SD, standard deviation; SSC, side scatter; Tot BM, total bone marrow.

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