GPVI deficiency, immunodepletion, or blockade reduced platelet adhesion to fibrin. (A) Representative DIC microscopy images of WT and GPVI−/− mouse platelets adhering to polymeric fibrin (300 s−1, 3 minutes). Bar, 30 µm. (B-C) Bar graph represents the quantification of adherent platelets. (B) Hirudinated mouse whole blood perfused over fibrin; WT (n = 7); GPVI−/− (n = 7); Ctrl: mice injected with a control antibody (n = 6); JAQ.1: WT mice injected with JAQ.1 (n = 5); hGPVI mouse whole blood treated with a control Fab (n = 6) or the Fab 9O12 (n = 6). (C) Human whole blood treated with a control Fab (n = 6) or the Fab 9O12 (n = 6) perfused through fibrin or FGN-coated microcapillaries (300 s−1, 3 minutes). Mean ± SEM, *P < .05, **P < .01, ***P < .001, Mann-Whitney U test. Ctrl, control; DIC, differential interference contrast; hGPVI, human GPVI.
