Blocking the early neutrophil response to sterile inflammation protected against prolonged elevation of peritoneal exudate cells in X-CGD. (A-D) WT and X-CGD mice were injected IV with neutralizing antibodies to either mouse IL-1α, G-CSF, IL-1β, or isotype control antibody, or with anti-Ly6G to deplete neutrophils, then challenged 1 hour with periodate i.p. and sacrificed after 72 hours. Total peritoneal leukocyte counts were enumerated. Data from 1 of 2 representative experiments (n = 3 mice per group) is presented as means ± standard deviation and statistical differences calculated using 1-way ANOVA with Tukey correction. *P < .05, **P < .01, ***P < .001. (E-F) Total number of macrophages (F4/80⁺) and neutrophils (Ly6G⁺) in the peritoneal exudates at 72 hours are shown. Each antibody-treated group was compared with a combined group of mice injected with periodate alone or with isotype + periodate as controls (▪). Statistical differences between controls and respective treatment groups were calculated using 1-way ANOVA with Dunnett correction. *P < .05, ϕP < .01, #P < .001; NS, nonsignificant.