Figure 3
Figure 3. Uptake of apoptotic tumor plasma cells by BM DCs and role of calreticulin in this process. (A) Representative micrographs of green fluorescent, immunomagnetically purified DCs (from MGUS and MM patients) that have engulfed, in vitro, γ-irradiated red fluorescent fragments of apoptotic plasma cells. (B-C) Changes in phagocytic activity of DCs in vitro, upon treatment with a CD91-blocking mAb, determined by flow cytometry. Representative plots (B) and percentages (C) of green DCs (from 9 MGUS and 9 MM patients) containing red apoptotic plasma cell fragments. Mann-Whitney U test. (D) Representative micrographs of immunomagnetically purified plasma cells (from MGUS and MM patients) showing membrane-associated fluorescence after surface staining with FITC-conjugated mAb to calreticulin. (E-F) Representative plots (E) and percentages (F) of tumor plasma cells (from 20 MGUS and 20 MM patients) that stained positively for surface calreticulin. Mann-Whitney U test. P values are shown only for statistically significant comparisons.

Uptake of apoptotic tumor plasma cells by BM DCs and role of calreticulin in this process. (A) Representative micrographs of green fluorescent, immunomagnetically purified DCs (from MGUS and MM patients) that have engulfed, in vitro, γ-irradiated red fluorescent fragments of apoptotic plasma cells. (B-C) Changes in phagocytic activity of DCs in vitro, upon treatment with a CD91-blocking mAb, determined by flow cytometry. Representative plots (B) and percentages (C) of green DCs (from 9 MGUS and 9 MM patients) containing red apoptotic plasma cell fragments. Mann-Whitney U test. (D) Representative micrographs of immunomagnetically purified plasma cells (from MGUS and MM patients) showing membrane-associated fluorescence after surface staining with FITC-conjugated mAb to calreticulin. (E-F) Representative plots (E) and percentages (F) of tumor plasma cells (from 20 MGUS and 20 MM patients) that stained positively for surface calreticulin. Mann-Whitney U test. P values are shown only for statistically significant comparisons.

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