BCL2 mutations at transformation in FL. (A) Overall frequency of BCL2 somatic mutations in FL validation cohort at diagnosis and transformation. Gray, blue, orange, and red depict samples without mutations, with only synonymous mutations, with mixed synonymous and nonsynomymous mutations, and with only nonsynonymous mutations, respectively. (B) Schematic diagram of the Bcl-2 protein showing Bcl-2 homology domains and distribution of new amino acid alterations for FL validation samples (n = 31) at transformation. Color-coded symbols depict distinct types of alterations, with purple for synonymous, white for nonsynonymous with no charge introduction, red for nonsynonymous with introduction of a negative charge, and blue for nonsynonymous with introduction of a positive charge. Red cross identifies a stop codon. (C) Depiction of bp preference of mutations in the FL validation at diagnosis and transformation (n = 31). The size of letters corresponds to the frequency with which that nucleotide is mutated in the BCL2 coding strand. bp, base pair; TM, transmembrane domain.