IFN-γ ELISPOT assay results. IFN-γ ELISPOT assay was performed using a commercially available kit according to the manufacturer’s instructions. PBMCs were resuspended in CTL medium and incubated for 48 hours (“Restore” protocol). Peptide-pulsed and irradiated T2 cells served as stimulator cells. Each error bar represents the standard deviation. (A) Representative subject UPN T64. (B) Representative subject UPN T14.