Flowchart of study. A dual-method approach consisting of an initial screening by genome-wide miRNA microarray of MCL samples from the MCL2 cohort, with subsequent confirmation of differentially expressed miRNAs by qRT-PCR in the same patients. Confirmed differentially expressed miRNAs identified in the MCL2 cohort were validated by qRT-PCR in samples from the MCL3 cohort. qRT-PCR, quantitative reverse-transcription polymerase chain reaction.