Survival of WT C57BL/6 mice expressing hFIX-WT and hFIX-Padua at supraphysiologic levels following AAV. Mice expressing hFIX-WT (gray lines) and hFIX-Padua (black lines) at levels resulting in (A) 100% to 230% activity and (B) 480% to 2020% activity did not have different survival rates. Black dotted line, empty capsid-treated control animals with normal FIX levels.