Generation of IBMI-huNOG mice and T-cell development in periphery. (A) Development of human leukocytes in bone marrow of IBMI-huNOG mice. Bone marrow cells from IBMI-huNOG mice (n = 20) at 1 mpt were analyzed by fluorescence-activated cell sorting (FACS) for expression of human CD45, CD19, and CD45, and mouse CD45 markers. Representatives (left) and the percentage of indicated markers (right) are shown. All cell populations were gated on mononuclear bone marrow cells. (B) Time course of human leukocyte development in the peripheral blood of IBMI-huNOG mice. Peripheral blood mononuclear cell (PBMC) from IBMI-huNOG mice (n = 40 for each time point) were stained for human CD45 at each time point. Box plots represent medians ± 1.5 IQR. (C) Increased number of human lymphocytes in IBMI-huNOG mice. Absolute numbers of human CD45⁺ and CD3⁺ cells in peripheral blood were determined by FACS analysis at each time point (n = 40 for each time point). (D) CD4-CD8 ratio in peripheral blood T cells. The CD4-CD8 ratio was calculated as follows: [(CD4 T-cell numbers per µL)/(CD8 T-cell numbers per µL)] (n = 40). (E) Sustained composition of human leukocytes in peripheral blood. PBMCs from IBMI-huNOG mice (n = 8) were stained for human CD45, CD3, and CD19. Results are presented as mean percentages of human CD45⁺ cells.