Figure 1 BMPR1 regulates MC effector... | American Society of Hematology

Figure 1

$Figure 1. BMPR1 regulates MC effector functions and increases MC survival. (A) MCs were stimulated with BMP4 at 20 ng/mL for the times indicated and the expression of selected MC marker genes quantified by reverse-transcription quantitative polymerase chain reaction and normalized to β-actin. Data are shown in relation to cells kept in the absence of bone morphogenetic protein (BMP) for the same times. (B) Cultured MCs (last addition of SCF 5-7 days earlier) were washed and replated in fresh media with or without BMP4 (20 ng/mL) for 24 hours, after which time histamine release was elicited by FcεRI crosslinking with different concentrations of AER-37. IgER, immunoglobulin E receptor. (C) Cultured MCs were stimulated by AER-37 (0.1 μg/mL) for 2 hours, washed, and replated in fresh media with or without BMP4 (20 ng/mL) ± stem cell factor (SCF) (100 ng/mL) for 48 hours. After this time, cells were subjected to a second round of stimulation by AER-37. MCs are largely refractory to a second stimulation for prolonged times, but BMP accelerates this recovery. Although SCF alone also accelerates recovery, the effects from BMP and SCF are additive. (D) MCs after isolation were cultured in the presence or absence of the indicated factors for 10 days, and recovery of viable cells was assessed. The numbers indicate growth factor concentrations in ng/mL. All data in this figure are the mean ± standard error of the mean from 5-7 independent assays. *P < .05; **P < .01; ***P < .001.$

BMPR1 regulates MC effector functions and increases MC survival. (A) MCs were stimulated with BMP4 at 20 ng/mL for the times indicated and the expression of selected MC marker genes quantified by reverse-transcription quantitative polymerase chain reaction and normalized to β-actin. Data are shown in relation to cells kept in the absence of bone morphogenetic protein (BMP) for the same times. (B) Cultured MCs (last addition of SCF 5-7 days earlier) were washed and replated in fresh media with or without BMP4 (20 ng/mL) for 24 hours, after which time histamine release was elicited by FcεRI crosslinking with different concentrations of AER-37. IgER, immunoglobulin E receptor. (C) Cultured MCs were stimulated by AER-37 (0.1 μg/mL) for 2 hours, washed, and replated in fresh media with or without BMP4 (20 ng/mL) ± stem cell factor (SCF) (100 ng/mL) for 48 hours. After this time, cells were subjected to a second round of stimulation by AER-37. MCs are largely refractory to a second stimulation for prolonged times, but BMP accelerates this recovery. Although SCF alone also accelerates recovery, the effects from BMP and SCF are additive. (D) MCs after isolation were cultured in the presence or absence of the indicated factors for 10 days, and recovery of viable cells was assessed. The numbers indicate growth factor concentrations in ng/mL. All data in this figure are the mean ± standard error of the mean from 5-7 independent assays. *P < .05; **P < .01; ***P < .001.

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