Patients with AAAs during the consolidation phase and in the first two weeks of the intensification. Serum AAAs against native E coli asparaginase (Coli-AAA levels) of patients at day 79 (start of the consolidation phase) and at day 140 (start of the intensification) (A), and Coli-AAA levels and serum AAAs against PEGasparaginase (PEG-AAA levels) at day 140 (before first PEGasparaginase dose) and at day 154 (14 days after first PEGasparaginase dose) (B-C). Dashed lines indicate that samples were defined as positive for AAAs if the Coli-AAA OD was >0.13 and if the PEG-AAA OD was >0.25. Closed circles and blocks show cases with allergy or cases without allergy and without silent inactivation; silent inactivation cases are shown by open circles and blocks. Median Coli-AAA and PEG-AAA levels are indicated by bars. NS, not significant.