IκB-ζ is highly expressed in ABC DLBCL and induced by NF-κB. (A) IκB-ζ is significantly higher expressed in ABC compared with GCB DLBCL (P = 5.7 × 10−37). Error bars depict the standard error of the mean. (B) IκB-ζ is highly expressed in 4 ABC DLBCL cell lines. In contrast, in other DLBCL cell lines, only low IκB-ζ expression is detectable by quantitative PCR. Error bars indicate the standard deviation. (C) Western blot analysis of IκB-ζ expression in DLBCL cell lines. Four of 5 ABC DLBCL cell lines have detectable IκB-ζ protein expression. (D) Expression of L265P MYD88 induces IκB-ζ expression in HT and U2932 measured by quantitative PCR and western blotting. (E) Expression of L244P CARD11 induces IκB-ζ expression in HT and U2932 measured by quantitative PCR and western blotting. **P < .01; ***P < .001.
