Osteocyte-specific loss of Gsα results in expansion of myeloid cells in BM and spleen. (A) Splenomegaly in OCY-GsαKO (KO) mice compared with controls (Con). (B) Representative areas of hypercellularity in BM of OCY-GsαKO femurs. (C) Decreased whole-body weight and (D) increased normalized spleen weights in OCY-GsαKO mice (n ≥ 7). (E-H) PB counts showing (E) number of leukocytes, (F) % neutrophils, (G) number of platelets, and (H) number of lymphocytes in 7- and 21-week-old control and OCY-GsαKO female mice (n ≥ 9). (I-L) Immunophenotypic analysis of hematopoietic cells in BM and spleen showing absolute counts for B cells (B220⁺ IgM⁺), granulocytes (Gr1⁺ CD11b⁺), erythroid cells (Ter119⁺ CD45⁻), monocytes (F4/80⁻ CD11b⁺), macrophages (F4/80⁺ CD11b⁺), T cells (CD4⁺), and cytotoxic T cells (CD8a⁺) in BM (I-J) and spleens (K-L) of 7-week-old (I,K) and 21-week-old (J,L) control and OCY-GsαKO female mice (n ≥ 6). Error bars represent mean ± SEM. *P < .05 by t test.