Analysis of thrombus formation in Pyk2-KO mice. (A) Photochemical-induced arterial thrombosis assesses as time required for occlusion of femoral artery measured by laser Doppler in WT (black bars) and Pyk2-KO (white bars) mice. Data are the means ± SD of measurements performed on 5 animals for both genotypes. ***P < .001. (B) Pulmonary thromboembolism–associated mortality caused by injection of epinephrine plus collagen in WT (black bars, 10 animals analyzed), and Pyk2-KO (white bars, 11 animals analyzed) mice. (C) Percentage of vessels occluded by platelet thrombi in the lungs of WT (black bars) and Pyk2-KO (white bars) mice on injection of collagen and epinephrine as determined by count in 10 microscopic fields for each lung section. Data are reported as the means ± SD. *P < .05. (D) Representative histology images (100×) of lungs from WT and Pyk2-KO mice after collagen plus epinephrine injection. Staining is with H&E. Arrows indicate the platelet-rich thrombi.