DN T cells are significantly increased in peripheral lymphoid organs. (A) The frequency of T-lymphocyte subsets in dLNs from WT and miR-146a TG mice at age 6 to 8 weeks. (B) The T-cell numbers of indicated lymphocyte subsets in dLNs (n = 4). (C) The expression of activation markers on CD4⁺ T cells from peripheral LNs by FACS analysis. (D) Increased CD3⁺CD4^-CD8^-TCRαβ⁺ DN T cells in the spleens from TG mice. Both the frequency and cell numbers were increased significantly in TG mice from 8 to 52 weeks. Results shown here are representative of at least 3 independent experiments (WT, n = 5; TG, n = 6). All data are expressed in terms of mean ± standard deviation values. Comparison of means was performed with an unpaired Student 2-tailed t test (**P < .01).