Regulated secretion of VWF and VWFpp from BOECs. BOECs derived from a healthy donor were cultured in 12-well plates until confluent. After rinsing with HBSS, cells were incubated for 60 minutes without (Control) or with (PMA) 160 nM PMA at 37°C. The regulated secretion of VWF (A) and VWFpp (B) was determined for BOECs at given passages. (C,D) Y-axis indicates the total production (medium plus lysate) of VWF or VWFpp by approximate 1 × 105 cells. Mean ± SEM indicate variation between triplicate measurements.