Multimer analysis of VWF. Multimers are shown for VWF in the plasma from indicated patients (A), in the culture medium (B), or in the cell lysates (C) of BOECs. The lanes separated by the black lines are representative results from the same gels or from different experiments. Normal pooled plasma (lane NPP) was used as reference. The plasma of the patient heterozygous for p.Cys2693Tyr was unfortunately no longer available, but the multimer pattern for this patient has been reported before as normal.²⁹  VWF multimer pattern of all healthy donors was normal; only 2 representative patterns are shown. The multimer patterns of VWF were analyzed by sodium dodecyl sulfate–agarose gel electrophoresis and Western blot under nonreducing conditions.