Figure 6
Figure 6. STAT5 regulates eIF2α downstream of JAK2V617F. (A) JAK2V617F-expressing FDC-P1–EPOR cells were treated with DMSO or STAT5 inhibitor (100 or 200μM for 1 hour) and CDC25A, P-eIF2α, eIF2α, P-STAT5, and STAT5 protein levels were analyzed by Western blot. (B) JAK2V617F-expressing FDC-P1–EPOR cells were transduced with control or STAT5 siRNA and CDC25A, P-eIF2α, eIF2α and STAT5 protein levels were analyzed by Western blot 8 hours after transfection. β-actin levels were used as control. Western blots are representative of 3 independent experiments.

STAT5 regulates eIF2α downstream of JAK2V617F. (A) JAK2V617F-expressing FDC-P1–EPOR cells were treated with DMSO or STAT5 inhibitor (100 or 200μM for 1 hour) and CDC25A, P-eIF2α, eIF2α, P-STAT5, and STAT5 protein levels were analyzed by Western blot. (B) JAK2V617F-expressing FDC-P1–EPOR cells were transduced with control or STAT5 siRNA and CDC25A, P-eIF2α, eIF2α and STAT5 protein levels were analyzed by Western blot 8 hours after transfection. β-actin levels were used as control. Western blots are representative of 3 independent experiments.

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