Figure 1 Clec1b −/− newborn pups are... | American Society of Hematology

Figure 1

$Figure 1. Clec1b−/− newborn pups are nonviable and visually distinct from Clec1b+/+ littermates at birth. (A) Blood-filled lymphatic vessels (black arrowhead) in the subcutaneous region of the skin persist at P0 (scale bar = 500 μm). (B) Clec1b−/− newborn pups that feed develop chylous ascites (black arrow) in the abdominal cavity (L indicates lobes of the liver; I, intestine; scale bar = 1 mm). (C) Example of flow cytometric analysis identifying LECs (podoplanin+CD31+) and BECs (podoplanin−CD31+) in the stromal fraction (CD45−Ter119−) of mesenteric vessel preparations from Clec1b+/+ (left) and Clec1b−/− (right) offspring. (D) Ratio of LECs to BECs in preparations of isolated mesenteric vessels (left; **P = .002 by unpaired t test; n = 3 Clec1b+/+; n = 5 Clec1b−/−) and intestine (right; **P = .012 by unpaired t test; n = 3 for each genotype) show significant decreases in P0 Clec1b−/− offspring.$

Clec1b^−/− newborn pups are nonviable and visually distinct from Clec1b^+/+ littermates at birth. (A) Blood-filled lymphatic vessels (black arrowhead) in the subcutaneous region of the skin persist at P0 (scale bar = 500 μm). (B) Clec1b^−/− newborn pups that feed develop chylous ascites (black arrow) in the abdominal cavity (L indicates lobes of the liver; I, intestine; scale bar = 1 mm). (C) Example of flow cytometric analysis identifying LECs (podoplanin⁺CD31⁺) and BECs (podoplanin⁻CD31⁺) in the stromal fraction (CD45⁻Ter119⁻) of mesenteric vessel preparations from Clec1b^+/+ (left) and Clec1b^−/− (right) offspring. (D) Ratio of LECs to BECs in preparations of isolated mesenteric vessels (left; **P = .002 by unpaired t test; n = 3 Clec1b^+/+; n = 5 Clec1b^−/−) and intestine (right; **P = .012 by unpaired t test; n = 3 for each genotype) show significant decreases in P0 Clec1b^−/− offspring.

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