RHAU^fl/fl:vav-iCre mice develop hemolytic anemia. (A) Increased erythropoietin level in blood plasma of RHAU^fl/fl:vav-iCre mice. *P < .05; n = 6 for each group. (B) Blood smear examination shows the presence of spherocytes (arrows) in RHAU^fl/fl:vav-iCre mice; (top) RHAU^fl/fl; (bottom) RHAU^fl/fl:vav-iCre. (C) Shortened lifespan of erythrocytes lacking RHAU. The half-lives of erythrocytes were determined by chasing biotinylated cells in vivo. Each dot represents the half-life value of erythrocytes from an individual mouse. The horizontal lines represent the average values, which are indicated at the bottom of the graph. *P < .05; n = 6 for RHAU^fl/fl and n = 5 for RHAU^fl/fl:vav-iCre.