Mevalonate rescues Rac1 farnesylation and activation and blood perfusion from inhibition by simvastatin. (A) Tissue extracts were prepared from ischemic and non-ischemic adductor muscles of WT mice that were administered with or without simvastatin in combination with and without mevalonate and analyzed for Rac1 farnesylation and activation as described in Figure 3. (B) Blood flow was measured by LDPI. (C) Ischemic adductor muscle sections of WT mice that were administered with or without simvastatin in combination with and without mevalonate were stained for CD31 (red) and vWF (green). The bar graphs in panels A and B represent the mean ± SD values of 3 independent experiments or 6 animals. *P < .05 versus WT non-ischemia; ^#P < .05 versus WT ischemia. **P < .05 versus WT ischemia + simvastatin.