Transplantation and homing of transduced murine hematopoietic progenitor cells. (A) Short-term engraftment of shRNA-transduced murine hematopoietic cells (YFP⁺) in the peripheral blood of recipient mice (n = 4-6 mice each). (B) Immunoblot of Hspa9 protein in transduced YFP⁺ cells isolated from the bone marrow and spleens of recipient mice 8 weeks after transplantation. (C) Transduction efficiency was measured as the percentage of YFP⁺ murine bone marrow progenitor cells after 2-4 days in culture (n = 3). (D) Homing efficiency of transduced cells was measured as the ratio of after-transplantation to before-transplantation YFP⁺ CFU-Cs (n = 4). *Nonspecific band in murine bone marrow and spleen cells. LUC indicates luciferase. All data represent the means ± SD.